We also characterized other genes that are regulated by ompR in this mutant, which contributed to bacterial antibiotic resistance. The loss of porins was due to incapability of phosphorylation, which is essential for porin transcription and carbapenem resistance. Moreover, we identified a novel mutation, G63S, in transcription factor OmpR which moderates the expression of outer membrane proteins. We sequenced and compared the transcriptomes of transconjugants that exhibited distinct carbapenem susceptibilities, and found that the loss of outer membrane proteins led to antibiotic resistance. After selection, transconjugants conferred varying minimum inhibitory concentrations with respect to carbapenems. ![]() coli J53 recipient cells and selected survivors using different concentrations of the broad spectrum antibiotic meropenem. In this study, we conjugated plasmid pNDM-HK to E. ![]() However, the response of plasmid-carrying bacteria to antibiotic treatment and how these bacteria evolve to increase their resistance remain elusive. ![]() Plasmids are one of the popular carriers to disseminate resistance genes among pathogens. Antibiotic resistance is an emerging public health issue.
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